Skip to main content
. Author manuscript; available in PMC: 2012 May 8.
Published in final edited form as: Cancer Res. 2011 May 24;71(11):3822–3830. doi: 10.1158/0008-5472.CAN-10-3782

Figure 4.

Figure 4

Clustering of Myc target gene expression in 2D and 3D cultures. A, hierarchical clustering analysis of Myc target expression by array and time point (2D MCF10A cells at day 2 or day 5, 3D MCF10A acini at day 8 or day 16). For each array, upregulated genes are represented in red and the downregulated genes are represented in green. Left bar, Myc target gene annotation. From the data set in the Myc Target Gene Database (2), each curated Myc target gene response is labeled in red (upregulated), green (downregulated), or gray (no annotation). B, Myc target gene validation. The 3D:2D ratio of transcripts for each Myc target gene was quantified by qRT-PCR relative to 18S rRNA. Expression of Myc, ornithine decarboxylase (ODC), eukaryotic translation initiation factor 4E (eIF4E), cyclin A2 (CCNA2), cyclin-dependent kinase 1/cell-division cycle 2 (CDK1/CDC2), ASF/SF2 (SFRS1), CDC6, and dihydrofolate reductase (DHFR) is shown. Error bars, ±1 SEM (for each bar, 99.5% confidence interval < 1; n = 3).