Table 1.
List of primers used for qRT-PCR analysis of gene expression.
| Primer | Sequence (5′ → 3′) | Position (mRNA) | Amplicon size (bp) | UPL probe no. | |
|---|---|---|---|---|---|
| HERP | FOR | ACTGTCGTTGCAGAGATTGC | 322 | 107 | 70 |
| HERP | REV | GTTCGGTCTCGGACTCCAT | 410 | ||
| GRP-78/Bip | FOR | TGAAGGTGAAAGACCCCTGA | 1654 | 107 | 15 |
| GRP-78/Bip | REV | CAAAGGTGACTTCAATCTGTGG | 1739 | ||
| EDEM-1 | FOR | GCTGAGTGATTACCTTAGCCACA | 5504 | 72 | 15 |
| EDEM-1 | REV | TGGACAGGAAAGCTCAGGAT | 5556 | ||
| WARS | FOR | CTGGACGGGTGGACTTAGC | 2667 | 77 | 74 |
| WARS | REV | GAGGCCTGCCTAGACATCTG | 2724 | ||
| PDI | FOR | TGAGGTCTGCGGAAGGTC | 2226 | 95 | 16 |
| PDI | REV | TCCGAGATACTGTCGTTTGTTG | 2299 | ||
| P58IPK | FOR | CTGAAGGAGCAAGAGACCATC | 2182 | 76 | 87 |
| P58IPK | REV | TTAATCCAAAGTCTAAGCCTGGATA | 2233 | ||
| BAX | FOR | TGACCCCTGACCTCACTGT | 763 | 82 | 29 |
| BAX | REV | TGAGCAATTCCAGAGGCAGT | 825 | ||
| CHOP | FOR | GTCATTGCCTTTCTCCTTCG | 192 | 61 | 33 |
| CHOP | REV | GTCCTCATACCAGGCTTCCA | 233 | ||
| ATF-4 | FOR | AAGGAGTTCGACTTGGATGC | 1156 | 78 | 85 |
| ATF-4 | REV | ATCCAACGTGGTCAGAAGGT | 1214 | ||
| CERS1 | FOR | CAATGTGGGCATCCTTGTG | 669 | 61 | 4 |
| CERS1 | REV | GGTGAACTCAAGCTGCACG | 711 | ||
| CERS2 | FOR | ACTTCTGGTGGGAACGTCTG | 370 | 73 | 27 |
| CERS2 | REV | ACACGTCCATCTCGGTCTTC | 423 | ||
| CERS4 | FOR | GCAGGTGATACACCACTTCG | 944 | 91 | 62 |
| CERS4 | REV | TAACAGCAGCACCAGAGAGC | 1015 | ||
| CERS5 | FOR | CATATGCGTGAGTTTCATTGC | 1440 | 95 | 57 |
| CERS5 | REV | GTGGAACTAAGGTGGGTTCAGT | 1513 | ||
| CERS6 | FOR | GCTGGTTTCGACAAAGACG | 468 | 60 | 2 |
| CERS6 | REV | TCTCACAGAACCTCGTCAGC | 508 | ||
| UGCG | FOR | GATCAGGTGGACCAAACTACG | 1109 | 71 | 4 |
| UGCG | REV | AGCATTCTGAAATTGGCTCAC | 1159 | ||
| GM3 Syn | FOR | TGCTGCTGATACTGGGTCTG | 1710 | 77 | 26 |
| GM3 Syn | REV | CAACAGAGAAGGGTTGTGACC | 1766 | ||
| SMPD-1 | FOR | GCACACCTGTCAATAGCTTCC | 1142 | 78 | 52 |
| SMPD-1 | REV | TGGCCATCGCTTCATAGAG | 1201 | ||
| SMPD-3 | FOR | AGGACCTGCTGTCTGCTGTC | 3956 | 76 | 79 |
| SMPD-3 | REV | TTGCAGCTCAATGGGTGAC | 4013 | ||
| SPTLC-1 | FOR | CGCGCTACTTGGAGAAAGA | 1324 | 77 | 52 |
| SPTLC-1 | REV | CTCTGTTTGTTCCACCGTGA | 1381 | ||
| SPTLC-2 | FOR | ATCGGTGTCGTTGTGGTTG | 1659 | 82 | 21 |
| SPTLC-2 | REV | TGGTATGAGCTGCTGACAGG | 1721 | ||
| CERD2 | FOR | GACCATGTCTGGACGAAGACTTC | 1626 | 77 | 34 |
| CERD2 | REV | CAACAGTCATGTTCTGCATCCC | 1681 | ||
| CERD3 | FOR | GGCAGTGGCTATAGCATATGG | 367 | 85 | 12 |
| CERD3 | REV | AGACCAGGCGGATGAACTG | 433 |
Abbreviations: CERS: ceramide synthase; SMPD: sphingomyelin phosphodiesterase; UGCG: UDP glucose ceramide glycosyltransferase; SPTLC: serine palmitoyl transferase; CERD: ceramidase; GM3Syn: GM3-synthase; EDEM: ER degradation-enhancing α-mannosidase-like protein; CHOP: C/EBP homologous protein; p58IPK: inhibitor of interferon-induced and double stranded RNA activated kinase; GRP78: glucose regulated protein 78; ATF: activating transcription factor-4; HERP: homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1; WARS: tryptophanyl-tRNA synthetase.