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. 2012 Apr 22;2012:479348. doi: 10.1155/2012/479348

Table 1.

List of primers used for qRT-PCR analysis of gene expression.

Primer Sequence (5′ → 3′) Position (mRNA) Amplicon size (bp) UPL probe no.
HERP FOR ACTGTCGTTGCAGAGATTGC 322 107 70
HERP REV GTTCGGTCTCGGACTCCAT 410
GRP-78/Bip FOR TGAAGGTGAAAGACCCCTGA 1654 107 15
GRP-78/Bip REV CAAAGGTGACTTCAATCTGTGG 1739
EDEM-1 FOR GCTGAGTGATTACCTTAGCCACA 5504 72 15
EDEM-1 REV TGGACAGGAAAGCTCAGGAT 5556
WARS FOR CTGGACGGGTGGACTTAGC 2667 77 74
WARS REV GAGGCCTGCCTAGACATCTG 2724
PDI FOR TGAGGTCTGCGGAAGGTC 2226 95 16
PDI REV TCCGAGATACTGTCGTTTGTTG 2299
P58IPK FOR CTGAAGGAGCAAGAGACCATC 2182 76 87
P58IPK REV TTAATCCAAAGTCTAAGCCTGGATA 2233
BAX FOR TGACCCCTGACCTCACTGT 763 82 29
BAX REV TGAGCAATTCCAGAGGCAGT 825
CHOP FOR GTCATTGCCTTTCTCCTTCG 192 61 33
CHOP REV GTCCTCATACCAGGCTTCCA 233
ATF-4 FOR AAGGAGTTCGACTTGGATGC 1156 78 85
ATF-4 REV ATCCAACGTGGTCAGAAGGT 1214
CERS1 FOR CAATGTGGGCATCCTTGTG 669 61 4
CERS1 REV GGTGAACTCAAGCTGCACG 711
CERS2 FOR ACTTCTGGTGGGAACGTCTG 370 73 27
CERS2 REV ACACGTCCATCTCGGTCTTC 423
CERS4 FOR GCAGGTGATACACCACTTCG 944 91 62
CERS4 REV TAACAGCAGCACCAGAGAGC 1015
CERS5 FOR CATATGCGTGAGTTTCATTGC 1440 95 57
CERS5 REV GTGGAACTAAGGTGGGTTCAGT 1513
CERS6 FOR GCTGGTTTCGACAAAGACG 468 60 2
CERS6 REV TCTCACAGAACCTCGTCAGC 508
UGCG FOR GATCAGGTGGACCAAACTACG 1109 71 4
UGCG REV AGCATTCTGAAATTGGCTCAC 1159
GM3 Syn FOR TGCTGCTGATACTGGGTCTG 1710 77 26
GM3 Syn REV CAACAGAGAAGGGTTGTGACC 1766
SMPD-1 FOR GCACACCTGTCAATAGCTTCC 1142 78 52
SMPD-1 REV TGGCCATCGCTTCATAGAG 1201
SMPD-3 FOR AGGACCTGCTGTCTGCTGTC 3956 76 79
SMPD-3 REV TTGCAGCTCAATGGGTGAC 4013
SPTLC-1 FOR CGCGCTACTTGGAGAAAGA 1324 77 52
SPTLC-1 REV CTCTGTTTGTTCCACCGTGA 1381
SPTLC-2 FOR ATCGGTGTCGTTGTGGTTG 1659 82 21
SPTLC-2 REV TGGTATGAGCTGCTGACAGG 1721
CERD2 FOR GACCATGTCTGGACGAAGACTTC 1626 77 34
CERD2 REV CAACAGTCATGTTCTGCATCCC 1681
CERD3 FOR GGCAGTGGCTATAGCATATGG 367 85 12
CERD3 REV AGACCAGGCGGATGAACTG 433

Abbreviations: CERS: ceramide synthase; SMPD: sphingomyelin phosphodiesterase; UGCG: UDP glucose ceramide glycosyltransferase; SPTLC: serine palmitoyl transferase; CERD: ceramidase; GM3Syn: GM3-synthase; EDEM: ER degradation-enhancing α-mannosidase-like protein; CHOP: C/EBP homologous protein; p58IPK: inhibitor of interferon-induced and double stranded RNA activated kinase; GRP78: glucose regulated protein 78; ATF: activating transcription factor-4; HERP: homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1; WARS: tryptophanyl-tRNA synthetase.