Table 3.
Control | Ethanol | P value | |
---|---|---|---|
Protein (Total) | |||
AKT | 18599.50 ± 744.87 | 22377.37 ± 478.47 | 0.0002 |
GSK3β | 2295.38 ± 250.09 | 1604.090 ± 208.06 | 0.017 |
PRAS40 | 462.13 ± 31.84 | 438.298 ± 15.13 | |
P70S6K | 1204.38 ± 130.96 | 1258.14 ± 170.01 | |
| |||
Protein (phospho) | |||
pS473-Akt | 273.47 ± 720.05 | 252.78 ± 33.53 | |
pS9-GSK3β | 612.40 ± 155.72 | 636.30 ± 46.82 | |
pT246-PRAS40 | 65.65 ± 5.42 | 78.07 ± 7.37 | |
pTpS421/424-p70S6K | 184.44 ± 23.30 | 114.32 ± 19.48 | |
| |||
Protein (Phospho/Total) ∗ 100 | |||
pS473-Akt/Akt | 1.43 ± 0.32 | 1.12 ± 0.14 | |
pS9-GSK3β/GSK3β | 28.42 ± 5.12 | 44.02 ± 8.15 | 0.038 |
pT246-PRAS40/PRAS40 | 14.29 ± 2.26 | 17.65 ± 1.46 | |
pTpS421/424-p70S6K/p70S6K | 13.91 ± 4.14 | 9.01 ± 0.97 |
Liver homogenates were used to measure immunoreactivity by multiplex ELISA. Phospho/total signaling protein levels were calculated. Immunoreactivity was normalized to protein concentration and data are expressed as mean ± SEM of fluorescence light units (arbitrary). Intergroup comparisons were made using t-tests. Significant P values are indicated in the right column.