Table 4.
Effects of chronic ethanol consumption on hepatic expression of proceramide genes-biosynthetic pathways.
| Protein | Control | Ethanol | P value |
|---|---|---|---|
| CerS1 | 0.542 ± 0.162 | 1.145 ± 0.228 | 0.025 |
| CerS2 | 3634 ± 509.2 | 3982 ± 450 | |
| CerS4 | 160.2 ± 36.36 | 172.7 ± 22.86 | |
| CerS5 | 1.62 ± 0.22 | 3.62 ± 0.27 | <0.0001 |
| CerS6 | 15.70 ± 3.59 | 33.52 ± 5.48 | 0.0071 |
| SPTLC-1 | 121.4 ± 9.29 | 253.3 ± 26.26 | 0.0001 |
| SPTLC-2 | 2615 ± 444.8 | 3187 ± 309.5 | |
| SMPD-1 | 685.7 ± 133.9 | 1214 ± 244.8 | 0.036 |
| SMPD-3 | 765.4 ± 140.5 | 1353 ± 216.6 | 0.019 |
| CERD2 | 6.524 ± 2.439 | 1.887 ± 0.3439 | 0.04 |
| CERD3 | 5.583 ± 1.458 | 6.896 ± 1.853 | |
| UGCG | 282.6 ± 68.27 | 609.9 ± 174.5 | 0.0006 |
| GM3Syn | 56.21 ± 7.80 | 72.32 ± 6.981 | 0.006 |
RNA extracted from normal or chronic alcoholic livers (N = 8/group) was reverse transcribed, and the cDNAs were used to measure gene expression by qPCR analysis with gene-specific primer pairs in a duplex qPCR reaction, in which the genes of interest were co-amplified with HPRT for normalization (see Section 2 and Table 1). Table represents relative levels of gene expression for Ceramide synthases (CERS), UDP glucose ceramide glycosyltransferase (UGCG), ceramidases (CERD), GM3-synthase, serine palmitoyl transferase subunits (SPTLC), sphingomyelinases (SMPD). Intergroup comparisons were made using Student's t-tests and significant P values are shown over the graphs.