Figure 1. Knockdown of UAP56 blocks Bcr induced DNA synthesis.

A. VSMC were transfected with UAP56 siRNA or control siRNA. 48 hours later the cells were treated with 50 MOI of Ad-Bcr or Ad-LacZ for 24 hours. During the last hour of incubation VSMC were pulse labeled with [³H] thymidine and incorporation of [³H] thymidine was measured. (**p<0.01). B. Western blots demonstrating Bcr and UAP56 expression in cells treated as in A. C. Following transfection with the indicated plasmids for 48 hours, HeLa cells were pulse labeled with [³H] thymidine for 1 hour. Cells were then harvested and [³H] thymidine incorporation was measured. (**p<0.01 compared with Wt-Bcr). pSG5 and pCMV6 are control plasmids for WT-Bcr and WT-UAP56 respectively. Results are means±SD. For all figures, the data are representative of triplicates using 2 or more different preparations of SMCs or HeLa cells.