Figure 4.

SUVH function affects rRNA gene cytosine methylation. (A) Diagram of the rRNA gene intergenic region highlighting the promoter region analyzed by bisulfite sequencing. (B) Histograms documenting the mean cytosine methylation levels at CG, CHG, or CHG motifs in wild type (Col-0) and suvh4, suvh5/6, and suvh4/5/6 mutants based on sequencing of at least 30 independent clones following bisulfite treatment of genomic DNA. The number of CG, CHG, or CHH motifs per clone is indicated (n). (C) Positions of methylcytosines in the promoter region, displayed according to sequence context: CG (red), CHG (blue), and CHH (green). The height of each bar denotes the frequency at which that cytosine is methylated.