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Virus yields of replication-competent HSV-1 mutants in various cell lines. Cells were seeded on 6-well plates at 5 × 105 cells per well. Triplicate wells were infected with R3616, R47Δ, G207, G47Δ, or strain F at an moi of 0.01. At 24 h after infection, cells were scraped into the medium and progeny virus was titered on Vero cells. In all cell lines tested, G47Δ showed a significantly higher replication capability than G207. Results represent the mean of triplicates ± SD.
