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. 2001 May 15;98(11):6396–6401. doi: 10.1073/pnas.101136398

Figure 4.

Figure 4

G47Δ precludes down-regulation of MHC class I expression in infected host cells. (a) Flow cytometric analyses of MHC class I expression in Detroit 551 human fibroblast cells 48 h after infection with HSV-1 (moi = 3). Whereas all HSVs with an intact α47 gene (wild-type strain F and G207) significantly down-regulated MHC class I expression, G47Δ completely precluded the down-regulation. (b) Time course of MHC class I down-regulation in Detroit 551 cells infected with HSV-1. For each virus, the peak value of MHC class I expression at 6, 24, or 48 h after infection, as analyzed by flow cytometry, was expressed as a percentage of the peak value of mock-infected cells (Control) at each time point. MHC class I down-regulation by G207 and R3616 occurred in a time-dependent fashion. Dissociation of MHC class I expression between α47-deleted mutants (G47Δ and R47Δ) and α47-intact viruses became apparent at 24–48 h after infection. (c) Flow cytometric analyses of MHC class I expression in human melanoma cell lines 24 h after infection with G207 and G47Δ. G47Δ caused a partial preclusion of MHC class I down-regulation in melanomas 1102 and 938, resulting in greater MHC class I expression than G207.