G47Δ-infected tumor cells stimulate T cells to a greater extent than
G207-infected tumor cells. Human melanoma cells were mock infected (no
virus) or infected with G207 or G47Δ at an moi of 3, and after 3–6
h, were cocultured with an equal number of responding human T cells for
18 h. T cell stimulation was assessed by an increase in IFN-γ
release into conditioned media. G47Δ-infected melanoma 1102 cells
caused a significantly greater stimulation of TIL888 cells compared
with G207-infected 1102 cells (P < 0.01, unpaired
t test). G47Δ-infected 938 melanoma cells also
stimulated TIL1413 cells, although the improvement was not
statistically significant compared with G207-infected 938 cells
(P = 0.1, unpaired t test). Neither
G207- nor G47Δ-infected melanoma 888 cells caused a significant
stimulation of TIL888 cells.