Figure 2.

Western blot results. 3T3-L1 cells were differentiated with insulin, dexamethasone and IBMX. Protein was isolated from whole cell extracts 0 h (T0) and 6 h (T6) after hormonal induction. 30 μg of each sample was subjected to Western blot analysis for eIF4B, IMPDH2, RPL27a and UBE2k/HIP2 expression. Changes in protein expression were quantified by densitometry and normalized with appropriate expression data of βActin. The values represent the average of three independent experiments, and the asterisk denotes a p-value < 0.05. eIF4B and RPL27a protein expression is 1.4 fold higher (p-value < 0.05) at T6 than time point T0. IMPDH2 and UBE2k/HIP2 show no significant differences in protein expression between T0 and T6.