Table 1.
Non specific binding responses of T.gondii and T. spiralis negative serum sets and conjugate alone
Serum | Bead identification | n | Min. response (MFI) | Max. response (MFI) | Mean response (MFI) | SE |
---|---|---|---|---|---|---|
Buffer | REF | 98 | 300 | 600 | 500 | 5 |
TOX | 98 | 3,900 | 4,700 | 4,300 | 19 | |
TRI | 98 | 3,200 | 3,900 | 3,500 | 17 | |
(T. gondii or T. spiralis) negative field sera | REF | 947 | 400 | 491,500 | 53,700 | 2,200 |
TOX | 932 | 4,200 | 269,200 | 47,900 | 1,100 | |
TRI | 945 | 3,400 | 505,500 | 75,300 | 2,300 | |
Experimental infection negative control sera | REF | 36 | 1,100 | 1,900 | 4,100 | 600 |
TOX | 36 | 4,900 | 45,500 | 21,500 | 1,900 | |
TRI | 36 | 4,200 | 34,800 | 10,400 | 1,000 |
Minimum, maximum and mean response signals of reference (REF), T. gondii (TOX) and T. spiralis (TRI) coupled beads after incubation of buffer, negative field sera (without positive responders in T. gondii ELISA) and sera of the negative control animals of the experimental infection. n, number of tested sera; SE, standard error