Table 2.

Infection status based specifications of bead-based assay and ELISA tests calculated by ROC analysis

	ROC analysis
					Maximum Youden Index
test	AUC	SE	P	95% CI	Cut-off	Se	Sp
BBA-TOX	0.911	0.015	< 0.001	0.881-0.940	13.90	0.86	0.96
E1-TOX	0.837	0.018	< 0.001	0.800-0.873	0.369	0.64	0.95
E2-TOX	0.865	0.018	< 0.001	0.829-0.901	0.174	0.76	0.93
E3-TOX	0.930	0.013	< 0.001	0.904-0.956	26.90	0.84	0.99
BBA-TRI	0.855	0.018	< 0.001	0.819-0.890	4.65	0.68	1.00
E-TRI	0.879	0.016	< 0.001	0.849-0.912	0.018	0.72	0.95

ROC analyses of a T. gondii and T. spiralis bead-based assay, three T. gondii and one T. spiralis ELISAs using responses of porcine serum samples from an experimental infection calculated against their infection status. AUC, area under the curve; SE, standard error of AUC; P, P-value; 95% CI, interval at a 95% confidence; Se, sensitivity; Sp, specificity; BBA-TOX, T. gondii antigen coupled bead-based assay; E1-TOX, RIVM in-house T. gondii ELISA, E2-TOX: Safepath T. gondii ELISA, E3-TOX, ID-VET T. gondii ELISA; BBA-TRI, T. spiralis antigen coupled bead-based assay and E-TRI, Safepath T. spiralis ELISA