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. 2012 May 7;209(5):1001–1010. doi: 10.1084/jem.20111675

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© 2012 Barr et al.

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Figure 2. — Mice with a B cell–specific IL-6 deficiency develop an attenuated form of EAE. (A) Splenic B cells (left) and non–B cells (right) from B-WT, B-IL-6^−/−, and IL-6^20% chimeric mice were MACS sorted and cultured for 72 h with PMA and ionomycin. Levels of IL-6 were then quantified by ELISA. Histograms represent the mean of triplicate cultures on pooled cells from 5–10 mice, with error bars indicating SEM. Data presented is representative of five separate experiments. (B) EAE progression was monitored for 32 d after immunization with MOG in B-WT, B-IL-6^−/−, and IL-6^20% chimeric mice. Each point represents the mean disease score and error bars represent SEM. Statistical analysis is by two-way ANOVA, where ***, P < 0.001, compared with B-WT mice. (C) Survival curves of chimeric mice after induction of EAE. Statistical analysis by log-rank test where NS, P > 0.05 and **, P < 0.01. Data presented is combined from three independent experiments (B-WT, n = 27; B-IL-6^−/−, n = 27; and IL-6^20%, n = 15).