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Detailed block diagrams of our proposed methods. Two dimensional (2D) version of the original 3D (A) Input image, (B) Pre-processed image, (C) Locally enhanced image (LEI) image; 2D version of the volume rendered images as (D) result of rough centroid extraction, (E) refined result after local shape analysis of LEI profiles, and (F) final result of centroid extraction after combining fragmented nuclei.
