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. 2012 May 8;7(5):e35550. doi: 10.1371/journal.pone.0035550

Table 1. Results for nuclei extraction at various stages of centroid extraction.

Number of nuclei
Proposed Method with Variant-1 (Variant-2) Ground truth
Image Stage-1 Stage-2 Stage-3 Nuclei
t12 44 (28) 22 (23) 17 (17) 17
t14 25 (21) 20 (20) 17 (17) 17
t40 114 (17) 16 (15) 16 (15) 17
t70 98 (26) 26 (24) 22 (18) 20
t85 77 (46) 37 (40) 30 (29) 30
t97 80 (31) 29 (27) 27 (25) 33

Number of estimated nuclei at various stages of the proposed method. Proposed method uses threshold parameters (Inline graphic = 0.97) in the rough extraction stage (Stage-1), (Inline graphic = 0.85) for the profile shape analysis (Stage-2), and (Inline graphic  = 15 pixels) for merging fragmented nuclei (Stage-3).