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. 2012 May 8;7(5):e36601. doi: 10.1371/journal.pone.0036601

Figure 7. NEMO-mediated recruitment of IKK1 and IKK2 to K13 and their activation by “T loop” phosphorylation.

Figure 7

A. Co-immunoprecipitation assay showing NEMO is essential for mediating the interaction of K13 with IKK1 and IKK2. FLAG-tagged K13 was immunoprecipitated from WT and NEMO-deficient Jurkat cells using control (C) and FLAG (F) antibody beads and presence of co-immunoprecipitated IKK1, IKK2 and NEMO detected by immunoblotting. B. MEFs expressing K13-ERTAM were treated with 4OHT and phosphorylation status of IKK1, IKK2 and IκBα was determined using Pathscan phospho-IKK1 (Ser176/180), phospho-IKK2 (Ser177/181), phosphor-IκBα (Ser32) ELISA kits, respectively.