Figure 1. Generation of Ptpn2^ex2+/ex2− mice.

(a) Ptpn2 genomic locus and targeting design. (b–c) Southern blot analysis of Ptpn2^ex2+/ex2+ (+/+) and Ptpn2^{ex2+/ex2 −} (+/−) mice. (b) Exon (Ex) 2 floxed mice mated with Oz-Cre mice for the germline deletion of exon 2 and removal of the Neomycin (Neo) resistance cassette. (c) Ex2-deleted mice were subsequently mated with C57BL/6 mice for the elimination of the Cre transgene. (d) PCR analysis of Ptpn2^ex2+/ex2+ (+/+), Ptpn2^{ex2+/ex2 −} (+/−) and Ptpn2^{ex2 −/ex2−} (−/−) mice. (e–f) Immunoblot analysis of TCPTP expression in lymphoid and non-lymphoid tissues detected with antibodies to the TCPTP non-catalytic C-terminus (6F3) and TCPTP N-terminus (6F7; raised against the first 38 residues of TCPTP); a non-specific (n.s.) protein detected by 6F7 is indicated. Results are representative of three independent experiments.