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. 2012 May 8;7(5):e36703. doi: 10.1371/journal.pone.0036703

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Wiede et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Representative longitudinal sections of tibiae from (a) 14 day-old Ptpn2⁻ ^/− (BALB/c), Ptpn2⁻ ^/− (C57BL/6) and Ptpn2^{ex2−/ex2−} mice, or (b) 21 day-old Ptpn2⁻ ^/− (BALB/c) and Ptpn2^{ex2−/ex2−} mice and their corresponding littermate wild type controls stained with the von Kossa technique (mineralized bone stained black). In a) safranin O staining [only shown for 14 day-old Ptpn2⁺ ^/+ (BALB/c) and Ptpn2⁻ ^/− (BALB/c)] highlights the delayed cartilage (in orange) destruction; scale bar = 100 micron.