Figure 7. Splenomegaly, myeloid development and lymphadenopathy in Ptpn2-deficient mice.

(a) Spleen weights from 18 day-old Ptpn2^{− /−} (BALB/c) and 28 day-old Ptpn2^{ex2−/ex2−} and Ptpn2^{− /−} (C57BL/6) mice and corresponding wild type littermate mice. (b) Splenocytes or (c) bone marrow (tibia and femur) cells were stained with fluorochrome-conjugated antibodies to CD11b and Gr1 and analysed by flow cytometry; absolute numbers of CD11b⁺, CD11b⁺Gr1⁺ and CD11b⁺Gr1⁻ were determined (d) Weights of pooled peripheral lymph nodes from 18 day-old Ptpn2^{− /−} (BALB/c) and 28 day-old Ptpn2^{ex2−/ex2−} and Ptpn2^{− /−} (C57BL/6) mice and corresponding wild type littermate mice. Results shown in (a–d) are means ± SEM for the indicated number of mice and are representative of at least two independent experiments; significance determined using a two-tailed Mann-Whitney U Test; *p<0.05 ** p<0.01 *** p<0.001.