Table 1. Summary of the intrinsic properties of ACTC mutant proteins related to the development of cardiomyopathies in humans.

	Tm	DNase-I	Nucleotide Release		Cc	Pi Release
ACTC	(°C)	IC50(nM)	Slow Rate (ms−1)	Fast Rate (ms−1)	(µM)	(s−1×10−2)
WT	56.8±1.3	13.2	1.12±2.64	17.47±7.40	0.28±0.13	1.24±0.02
E99K	68.8±0.35*	25.7	2.24±2.18	17.73±5.76	1.67±0.43*	1.25±0.02
	56.7±0.23†
Y166C	54.1±1.9	24.6	1.62±0.61	16.01±4.56	2.67±0.45*	0.38±0.02*
A230V	53.9±1.9	15.3	2.02±0.42	16.25±4.16	2.11±1.23	1.51±0.24
M305L	55.5±1.7	17.0	2.21±1.94	18.23±1.61	2.21±1.78	2.43±0.03*
R312H	53.6±2.0	36.7	8.87±5.52	181.8±140.7**	4.75±0.27*	2.59±0.05*
A331P	57.4±0.23	26.0	3.63±4.21	22.75±9.05	2.95±1.70	0.88±0.06*
E361G	55.6±1.7	16.8	2.06±1.35	18.11±2.97	0.58±0.48	0.66±0.05*

^†labeled with tetramethylrhodamine.

^* p<0.05 by t–test.

^** p<0.15 by t–test.

All values are the average of at least three replicates, showing the standard deviation of each.