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. 2012 May 8;7(5):e36570. doi: 10.1371/journal.pone.0036570

Table 1. Identification of founder sequences from EPD-PCR and bulk-PCR clone sequence alignments.

Individual EPD network Clone network
EPD-PCRhaplotypes Number ofsequences HaplotypeOP Bulk-PCRclonehaplotypes HaplotypeOP Number and originof sequences#
B1* 1 13 0,93 a 0,36 1×B
b 0,26 3×C
c 0,1 2×A
2 1 1 d* 0,5 2×E
3 1 0,07
B2 1 11 0,88 a 0,48 2×A, 4×B, 2×C, 1×D, 2×E
b 0,35 1×E
2 1 0,03
3 1 0,03
4 1 0,03
5 1 0,03
B3 1 12 0,9 a 0,33 3×B, 1×C, 2×D, 2×E
b 0,23 1×C
c 0,23 1×D
2 1 0,03
3 1 0,03
4 1 0,03
B4 1 9 0,6 a 0,35 1×B, 1×C, 1×E
2 6 0,4 b 0,26 2×D, 1×E
c 0,15 2×A
T1* 1 10 0,9 a 0,37 1×E
2 4 1 b 0,11 1×A, 1×D
c 0,07 1×E
d 0,07 1×C
e 0,04 1×B, 1×D
f 0,04 2×A
3 1 0,09
T2* 1 10 0,89 a 0,33 1×B, 3×D, 4×E
b 0,26 2×B
c 0,23 1×A
2 1 0,03
3 1 0,03
4 1 0,03
5 2 1
T3 1 14 0,93 a 0,29 2×C, 2×D, 2×E
b 0,17 1×B
c 0,17 1×B
d 0,17 1×E
2 1 0,07
T4 1 7 0,5 a 0,32 2×A
2 6 0,44 b 0,19 2×D, 1×E
c 0,05 1×E
3 1 0,03
4 1 0,03
T5 2 6 0,4 a 0,33 1×C, 1×E
1 9 0,6 b 0,22 2×B, 2×D
c 0,11 1×E
T6* 1 7 0,88 a 0,74 2×A, 2×B, 1×C, 2×D, 2×E
2 1 0,13
3 7 1

All EPD-PCR haplotypes are presented, while only those haplotypes getting OP values above the minimum value are shown for bulk-PCR clone sequences. Assumed founder sequences are highlighted in bold. # origin of sequences refer to the bulk-PCR products from which they originate (e.g., if haplotype a appeared two times in PCR product B and once in PCR product C, then 2×B and 1×C will appear in this column) * individuals for which statistical parsimony analyses produced two separated networks; here the sum of all OPs will be greater than one as OPs will be calculated independently for each network.