Table 1. Identification of founder sequences from EPD-PCR and bulk-PCR clone sequence alignments.
Individual | EPD network | Clone network | ||||
EPD-PCRhaplotypes | Number ofsequences | HaplotypeOP | Bulk-PCRclonehaplotypes | HaplotypeOP | Number and originof sequences# | |
B1* | 1 | 13 | 0,93 | a | 0,36 | 1×B |
− | − | − | b | 0,26 | 3×C | |
− | − | − | c | 0,1 | 2×A | |
2 | 1 | 1 | d* | 0,5 | 2×E | |
3 | 1 | 0,07 | − | − | − | |
B2 | 1 | 11 | 0,88 | a | 0,48 | 2×A, 4×B, 2×C, 1×D, 2×E |
− | − | − | b | 0,35 | 1×E | |
2 | 1 | 0,03 | − | − | − | |
3 | 1 | 0,03 | − | − | − | |
4 | 1 | 0,03 | − | − | − | |
5 | 1 | 0,03 | − | − | − | |
B3 | 1 | 12 | 0,9 | a | 0,33 | 3×B, 1×C, 2×D, 2×E |
− | − | − | b | 0,23 | 1×C | |
− | − | − | c | 0,23 | 1×D | |
2 | 1 | 0,03 | − | − | − | |
3 | 1 | 0,03 | − | − | − | |
4 | 1 | 0,03 | − | − | − | |
B4 | 1 | 9 | 0,6 | a | 0,35 | 1×B, 1×C, 1×E |
2 | 6 | 0,4 | b | 0,26 | 2×D, 1×E | |
− | − | − | c | 0,15 | 2×A | |
T1* | 1 | 10 | 0,9 | a | 0,37 | 1×E |
2 | 4 | 1 | b | 0,11 | 1×A, 1×D | |
− | − | − | c | 0,07 | 1×E | |
− | − | − | d | 0,07 | 1×C | |
− | − | − | e | 0,04 | 1×B, 1×D | |
− | − | f | 0,04 | 2×A | ||
3 | 1 | 0,09 | − | − | − | |
T2* | 1 | 10 | 0,89 | a | 0,33 | 1×B, 3×D, 4×E |
− | − | − | b | 0,26 | 2×B | |
− | − | − | c | 0,23 | 1×A | |
2 | 1 | 0,03 | − | − | − | |
3 | 1 | 0,03 | − | − | − | |
4 | 1 | 0,03 | − | − | − | |
5 | 2 | 1 | − | − | − | |
T3 | 1 | 14 | 0,93 | a | 0,29 | 2×C, 2×D, 2×E |
− | − | − | b | 0,17 | 1×B | |
− | − | − | c | 0,17 | 1×B | |
− | − | − | d | 0,17 | 1×E | |
2 | 1 | 0,07 | − | − | − | |
T4 | 1 | 7 | 0,5 | a | 0,32 | 2×A |
2 | 6 | 0,44 | b | 0,19 | 2×D, 1×E | |
− | − | − | c | 0,05 | 1×E | |
3 | 1 | 0,03 | − | − | − | |
4 | 1 | 0,03 | − | − | − | |
T5 | 2 | 6 | 0,4 | a | 0,33 | 1×C, 1×E |
1 | 9 | 0,6 | b | 0,22 | 2×B, 2×D | |
− | − | − | c | 0,11 | 1×E | |
T6* | 1 | 7 | 0,88 | a | 0,74 | 2×A, 2×B, 1×C, 2×D, 2×E |
2 | 1 | 0,13 | − | − | − | |
3 | 7 | 1 | − | − | − |
All EPD-PCR haplotypes are presented, while only those haplotypes getting OP values above the minimum value are shown for bulk-PCR clone sequences. Assumed founder sequences are highlighted in bold. # origin of sequences refer to the bulk-PCR products from which they originate (e.g., if haplotype a appeared two times in PCR product B and once in PCR product C, then 2×B and 1×C will appear in this column) * individuals for which statistical parsimony analyses produced two separated networks; here the sum of all OPs will be greater than one as OPs will be calculated independently for each network.