Table 2. E. histolytica cyst proteins detected in 3 or more cyst samples.

E. histolytica Locus ID	*Detected in samples	Annotation	Transcriptome data**			E. dispar homolog
			p-value	fold change: cyst/troph	HM-1 expr	Locus ID	% identity
EHI_012500	3, 4, 5	coatomer gamma subunit, putative	0.0881	−2.7	1.90	EDI_129270	92
EHI_038630	2, 3, 4, 5	TBC domain containing protein, putative	0.3190	1.7	0.06	EDI_259490	91
EHI_019630	1, 4, 5	hypothetical protein	0.0001	515.8	0.08	EDI_199570	54
200.m00090	1, 4, 5	clathrin heavy chain, putative	0.7530	−1.0	32.75	EDI_237980	92
EHI_109890	1, 3, 4, 5	chitinase, putative	0.0001	87.4	0.20	EDI_120190	82
EHI_152170	1, 3, 4, 5	chitinase Jessie 3, putative	0.0001	43.5	0.67	EDI_038370	90
EHI_146120	1, 2, 4	hypothetical protein	0.0001	59.1	0.04	EDI_105270	65
EHI_196570	1, 2, 3, 4, 5	hypothetical protein	0.0001	37.2	0.55	EDI_039770	93

*: Detected in samples: 1 = 8076; 2 = 4268; 3 = AM951; 4 = AM797; and 5 = CMS33-7132;

**: From [22]; NF = not found; troph = trophozoite; expr = expression; N/A = Not applicable; shown in bold fonts are those that show 90% or less identity with E. dispar proteins; ideal candidates for E. histolytica specific diagnostic tool development. NB. None of the proteins listed in Table 2 could be detected in any of the 4 trophozoite-specific proteome datasets available at the AmoebaDB or EST datasets (as of February, 2012).