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Incorporation of tritium label into CTP, UTP, and nucleic acids in the T. brucei brucei cell line TC221. [3H]Uracil was added to cultivated bloodstream forms, and aliquots were collected and analyzed at various times (see Materials and Methods). (A) Incorporation of tritium into UTP (▴) and CTP (■). The values are plotted as specific activities (dpm/nmol of each nucleotide). (B) Incorporation of label into RNA + DNA (■) and DNA only (▴), shown as absolute values (dpm).
