Figure 4. Elevated Ca spark frequency but similar SR Ca content in intact myocytes from WT and AnkB+/− mice in the presence of isoproterenol.
(A) Representative example of Ca sparks measurements in the presence of 1 μM ISO in an AnkB+/− myocyte. (B) Expanded scale for the areas marked with a white box in panel A. (C) Ca spark frequency in myocytes from WT and AnkB+/− mice pre-conditioned by pacing at 0.5, 1 and 2 Hz in the presence of 1 μM ISO. (D) SR Ca content in myocytes from WT and AnkB+/− mice paced at 0.5, 1 and 2 Hz in the presence of 1 μM ISO. For each pacing frequency, n=10 cells from 4–6 mice for both groups. *P<0.05, **P<0.01.