Table I.
CP libraries. CP libraries are described by their guest residue composition, number of pentapeptide repeats and the theoretical molecular weight of the CP incorporating both the leader (Ser-Lys-Gly-Pro-Gly) and the trailer [Cys(Gly-Gly-Cys)7] sequences. CPs 1–4 are listed in order of increasing hydrophobicity.
| Xaa (A:V) | Pentamers | MW (Da) | Expressed | |
|---|---|---|---|---|
| CP1 | 1:0 | 5 | 4315.8 | |
| 10 | 6223 | |||
| 20 | 10037.3 | |||
| 40 | 17665.9 | * | ||
| 80 | 32923.2 | * | ||
| 160 | 63437.8 | * | ||
| CP2 | 14:1 | 15 | 8158.2 | |
| 30 | 13907.7 | |||
| 60 | 25406.8 | * | ||
| 120 | 48404.9 | |||
| 150 | 59904 | * | ||
| CP3 | 9:1 | 10 | 6251 | |
| 20 | 10093.4 | |||
| 40 | 17778.2 | * | ||
| 80 | 33147.6 | * | ||
| 160 | 63886.6 | * | ||
| CP4 | 4:1 | 5 | 4343.9 | |
| 10 | 6279.1 | |||
| 20 | 10149.5 | |||
| 40 | 17890.4 | * | ||
| 80 | 33372.1 | * | ||
| 160 | 64335.5 | * | ||
The * indicates CPs that were expressed in E. coli from their plasmid-borne synthetic gene.