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. Author manuscript; available in PMC: 2013 Apr 6.
Published in final edited form as: Cell Immunol. 2012 Apr 6;275(1-2):19–23. doi: 10.1016/j.cellimm.2012.04.001

Fig 1. AHCC induces expression of IL-1β in human monocytes.

Fig 1

Monocytes purified from peripheral blood of healthy individuals were incubated for 5 hours with AHCC freeze dried powder (AHCC FD, 500 μg/ml for A and B), α-glucan fraction (500 μg/ml) of AHCC, LPS (100 ng/ml) or vehicle control (control) in the presence or absence of the LPS neutralizer polymyxin B (50 μg/ml). Cells were then fixed, permeabilized and stained with antibodies to IL-1β. Stained cells were analyzed on a flow cytometer. (A) Representative dot plots showing IL-1β expression by monocytes. (B) Polymyxin B (50 μg/ml) inhibited IL-1β expression by monocytes treated with LPS but not AHCC FD (500 μg/ml). Representative data from 6 (A) and 5 (B) independent experiments.