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. Author manuscript; available in PMC: 2013 Apr 6.
Published in final edited form as: Cell Immunol. 2012 Apr 6;275(1-2):19–23. doi: 10.1016/j.cellimm.2012.04.001

Fig 3. AHCC-treated monocytes induce high levels of IL-17 and IFN-γ production from CD4+ T cells in the presence of anti-CD3 and -CD28 antibodies.

Fig 3

Purified human monocytes that had been treated for 5 hours with AHCC FD at high (AHCC FD-H, 500 μg/ml) or low (AHCC FD-L, 100 μg/ml) concentrations, LPS (100 ng/ml) or vehicle control were added to autologous memory CD4+ T cells. Cells were then cultured for 7 days in the presence of anti-CD3 and –CD28 antibodies. Culture supernatants were collected and analyzed for IL-17, IFN-γ and IL-13 by multiplex cytokine assay. Bars and error bars indicate mean (n = 8) and standard error of mean, respectively. *P < 0.05.