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A. Effect of AC isoforms on HCN2RE fractional activation (n=6–8, p>0.05 by Two Way ANOVA). B. Effect of AC1 or AC6 co-expression with mHCN2 or mHCN2RE on the spontaneous beating rate of 4-day old NRVM cultures. *,# p<0.05 versus corresponding GFP-control (One Way ANOVA).