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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Mol Cell Cardiol. 2012 Mar 29;52(6):1233–1239. doi: 10.1016/j.yjmcc.2012.03.010

Figure 6.

Figure 6

Midpoint of activation (V50) of mHCN2 and its responsiveness to β-adrenergic receptor agonist - isoproterenol (ISO, 1μM). Cells were pre-treated with 5 μM BAPTA-AM for 8 minutes; ISO perfusion was started 1 minute before recording. Two Way ANOVA was used for group comparison where ISO midpoint values were compared in relation to the presence/absence of BAPTA in AC1 or AC6 groups. *p<0.05 versus corresponding no ISO; n=5–9.