Figure 5.
Mitochondrial localization of fluorescently-labeled TPP-PEG-L compared to PL by confocal laser scanning microscopy. HeLa cells were incubated with Rh-PE-labeled TPP-PEG-L-8% and PL for 18 h and then stained with MTG and Hoechst 33342. Yellow spots in the merged pictures denote the co-localization of the liposomes within mitochondrial compartments. A. Bright field images; B. Images of nuclei stained with Hoechst; C. Mitochondria staining with MTG; D. Uptake of Rh-labeled liposomes; E. Merged picture without DIC; F. Merged picture of all (A).