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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Apr 8;19(5):525–S1. doi: 10.1038/nsmb.2277

Figure 5.

Figure 5

Cl−-coupled H+ binding to the E203Q mutant. (a-c): Panels as in Figure 2 with the difference that 75-100 mM KCl is titrated into the experimental chamber. The averaged thermodynamic parameters are reported in Table 1. (d) Plot of the total enthalpy of Cl− binding, ΔHtot, as a function of the enthalpy of buffer ionization, ΔHbuff. Squares: experimental data. Solid line: fit to Eq. 1 with ΔHprot = −5.4±0.3 kcal mol−1 and N = 0.43±0.05. The errors for the experimental points are the s.e.m. while those on ΔHprot and N represent the uncertainty of the fit.