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. 2011 Jun 28;36(5):725–734. doi: 10.1038/ijo.2011.124

Figure 3.

Figure 3

Effects of BMP-3b knockdown and overexpression on adipogenesis. (a) Effects of siRNA-mediated BMP-3b knockdown on BMP-3b expression on day 0 and day 8, and quantitative RT-PCR analysis of adipogenic markers on day 8. Relative gene expression is shown as the ratio compared with the control. si, siRNA. (b) Effects of overexpressing the BMP-3b gene. Quantitative RT-PCR analysis of BMP-3b on day 0 and day 8, and analysis of adipogenic markers on day 8. The expression levels were normalized to S18 levels. Relative gene expression is shown as the ratio compared with the control expression level. The data are expressed using a logarithm scale for BMP-3b. Mock, plasmid vector control. The data are presented as the means±s.e.m. (n=3). **P<0.01 versus control or mock. Experiments were performed three times with independent cultures.