Figure 4.

Radioresistant mast cells were involved in suppression of neutrophil recruitment by Tolerimod1 treatment. (a) WT, (b) Tlr7^−/−, or chimeric mice [WT→ Tlr7^−/− (c), and Tlr7^−/−→WT (d)] were treated with 200 nmol Tolerimod1 (TLM1) s.c. for 3 days and peritonitis was induced with TG (n = 4 to 8 per group). (e) Mast cell-deficient Kit ^W-sh mice or Kit ^W-sh mice reconstituted with WT mast cells were treated with 200 nmol Tolerimod1 (TLM1) s.c. for 3 days, and peritonitis was induced with i.p. TG. The peritoneal cells were recovered by lavage after 3 hours and quantitated. Data shown are mean ± SEM and are pooled from 2 independent experiments that showed similar trends. P values are from comparisons of vehicle (veh)- and Tolerimod1-treated groups by Mann-Whitney test.