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. Author manuscript; available in PMC: 2013 May 8.
Published in final edited form as: Biochemistry. 2012 Apr 24;51(18):3933–3940. doi: 10.1021/bi201881p

Figure 2.

Figure 2

Michaelis-Menten kinetics of MID1-zinc hydrolysis of 4NPA. A fit of kobs versus [4NPA] to a standard binding equation indicates a kcat of 0.22 ± 0.01 s−1 and a KM of 0.47 ± 0.07 mM. Rates were determined at 25°C in the presence of 2.5 µM MID1-zinc buffered with 40 mM HEPES pH 8.5 and 50 mM NaCl.