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. Author manuscript; available in PMC: 2012 May 9.
Published in final edited form as: Cancer Gene Ther. 2011 Jul 15;18(9):663–673. doi: 10.1038/cgt.2011.37

Figure 3. DC.IL32 promote enhanced Type-1 polarization, but not proliferation, of alloreactive CD8+ T cells in vitro.

Figure 3

In A., CFSE-labeled (H-2d) CD8+ splenocytes were stimulated with H-2b control DC (DC.null or DC.ψ5) or DC.IL32for 3 days as outlined in Materials and Methods. Cells were then harvested and CD8+ gated cells analyzed for CSFE fluorescence dilution by flow cytometry. In B., the percentage of proliferating CD8+ T cells in each of the indicated cultures is reported as mean ± SD. Cell-free supernatants were also harvested from these DC-T cell cultures and analyzed for IFN-γ and IL-10 concentrations using commercial ELISA (panel C). Data are reported as mean ± SD from triplicate determinations. All experiments are representative of data obtained in 3 independent experiments. NS = Not significant; *p < 0.05; **p < 0.01.