Figure 1.
Targeting regulated transgene expression to the neuroepithelium. (a) Targeting vector used to generate PnestinrtTA/− mice. (b) Whole-mount X-gal staining is restricted to the CNS in an E12 PnestinrtTA/− mouse. TE, telencephalon; ME, mesencephalon; RH, rhombencephalon; SC, spinal cord; arrowhead, retinal primordium; and arrow, trigeminal ganglion. (c) No labeling was seen in a WT littermate. (d) The neuroepithelia of the cerebral cortex (CC), lateral ganglionic eminence (LGE), and hippocampus (HI) in a histological section. LV, lateral ventricle, ST, striatum. (Scale bars: a and b = 1 mm; c = 500 μm.)