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. Author manuscript; available in PMC: 2013 May 4.
Published in final edited form as: Cell Stem Cell. 2012 May 4;10(5):520–530. doi: 10.1016/j.stem.2012.04.007

Figure 1. Constitutively increased Cdc42 activity results in premature aging of young HSCs.

Figure 1

(A), Scheme of the experimental set-up for the competitive BM transplants. (B), Contribution of total donor-derived Ly5.2+ cells in PB after 20 weeks in competitive primary and secondary transplants. Percentages of engrafted cells are normalized according to stem cell equivalent. Mice were considered as engrafted when the percentage of Ly5.2+ cells in PB was higher than 1.0 and contribution was detected for all PB lineages. (C–D), Contribution of B-cells, T-cells and myeloid cells among donor-derived Ly5.2+ cells in PB after 20 weeks in competitive primary (C) and secondary (D) transplants. (E–G), Representative FACS dot plots (E) and quantitative and statistical analysis of LT-HSCs, ST-HSCs and LMPPs distribution among donor-derived LSKs in primary (F) and secondary (G) transplanted mice. * P < 0.05, ** P < 0.01, *** P < 0.001; columns are mean +1 S.E. The experiment was repeated three times with a cohort of 4 to 5 recipient mice per group (n = 14). See also Figure S1.