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. 2012 Apr 24;2012:356412. doi: 10.1155/2012/356412

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Copyright © 2012 Mary J. Eaton et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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hNT2.17 and hNT2.19 cells are nontumorigenic: BrdU stain. hNT2.17 (a–c) and hNT2.19 (d–f) cells were exposed to 1 μM bromodeoxyuridine (BrdU) (a, d) during 3 days of proliferation or (b, c, e, f) for 1 week during differentiation in vitro. Proliferating cells (a, d) incorporate abundant BrdU during proliferation. Viable differentiated cells (b, e) were labeled with DAPI (4′6-diamidino-2-phenylindal-2HCl) stain, while the same field (c, f) of differentiated cells did not incorporate any BrdU during differentiation. After 2 weeks of treatment with retinoic acid (RA) and mitotic inhibitors, hNT2.17 and hNT2.19 cells cease dividing and differentiation proceeds without further cell division. Magnification bar = 20 nm (d); 30 nm (a, b, c, e, f).