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. Author manuscript; available in PMC: 2012 May 9.
Published in final edited form as: J Cell Biochem. 2009 Dec 15;108(6):1389–1398. doi: 10.1002/jcb.22371

Figure 2. PDEF expression results in altered cell cycle progression and expression of cell cycle regulatory genes.

Figure 2

(A) Cell cycle analysis of untreated DLD-1 cells or cells treated with Ad-GFP or Ad-PDEF and harvested 36 hours after infection. (B) Western blot analysis of total protein isolated from cells infected with Ad-GFP or Ad-PDEF using antibodies against the indicated cell cycle proteins. Blots were re-hybridized using antibodies against PDEF and β-Actin. Control lanes represent uninfected cells. (C) PDEF is bound to the p21 expression in vivo. Chromatin immuno-precipitation (CHIP) analysis using chromatin prepared from the HT-29 cell line. Q-PCR analysis using primers for the p21 promoter and chromatin immunoprecipitation with a PDEF-specific antibody, compared to IgG control antibody.