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. Author manuscript; available in PMC: 2012 May 9.
Published in final edited form as: J Cell Biochem. 2009 Dec 15;108(6):1389–1398. doi: 10.1002/jcb.22371

Figure 4. PDEF expression levels alter cell migration.

Figure 4

The effect of PDEF expression on the migration of colon cancer cells was measured by transwell assay. (A) DLD-1 cells were untreated or infected with Ad-GFP or Ad-PDEF and migration measured by transwell assays. Data is representative of three experiments performed in triplicate. (B) LoVo, CaCo-2, and SW620 cells were infected with Ad-GFP or Ad-PDEF. Cell migration was measured by transwell migration assay. (C) Western blot analysis of PDEF protein expression in HT-29 colon cancer cells and in HT-29 expressing short hairpin RNA (shRNA) PDEF and control vectors. Actin levels provided as a control. (D) Cell migration of HT-29 colon cancer cells and of HT-29 cells expressing PDEF shRNA. Each of the measured differences were statistically significant with a p-value <0.05 by students t-test.