Figure 4.

Homeostatic scaling of mEPSC amplitude produced by chronic application of bicuculline or tetrodotoxin is both mimicked and occluded by altered Rpt6 phosphorylation. A, Example traces of mEPSCs recorded from hippocampal neurons expressing Rpt6 WT, S120A, or S120D. Calibration: 10 pA, 250 ms. B, S120A Rpt6 increases while S120D decreases mEPSC amplitude relative to WT, respectively. C, No significant change to mEPSC frequency was observed. n = 8, 12, and 9, respectively, for WT, S120A, and S120D; *p < 0.05, ANOVA with post hoc Fisher's least significant difference (LSD) test. D, Rpt6 S120A mimics and occludes scaling of mEPSC amplitude produced by chronic application of TTX (2 μm, 24 h) and BIC (50 μm, 48 h), respectively. Conversely, Rpt6 S120D mimics and occludes scaling of mEPSC amplitude produced by chronic application of BIC and TTX, respectively. n = 14–29 cells per condition. For comparison of untreated, TTX, and BIC: *p < 0.05, ANOVA and post hoc Fisher's LSD test. For comparison of S120A or S120D with or without TTX and BIC, respectively: p > 0.05, ANOVA. NS, No significant difference in bracketed conditions. Note: S120A and S120D alone conditions in D are taken from B for comparison purposes.