Figure 8.

Flow cytometry: brains. Representative bivariate plots show the relative numbers of CD4⁺ and CD8⁺ T-cells (A) and activated microglia (CD45^hiCD11b^hi) (B) in brains of untreated wt, untreated mut, AAV-mut, and AAV+BMT-mut groups. Quantitation of T-cells shows that there is a significant increase in CD4 and CD8 T-cells (C, D) in the AAV-mut group compared with the untreated wt. There is no increase in CD4 and CD8 T-cells in the untreated mut and AAV+BMT-mut groups compared with the untreated wt group (B–D). There appears to be an increase in CD4, CD8, and activated microglia (CD45^hiCD11b^hi) in the AAV-mut group compared with other groups. Quantitation of resting microglia (CD45^loCD11b^hi) (E) shows that there is a significant decrease in these cell numbers in the untreated mut and AAV+BMT-mut groups compared with the untreated wt group. Quantitation of activated microglia (F) shows that there is a significant increase in these cell numbers in the AAV-mut group compared with the untreated wt or AAV+BMT-mut group. There is no significant difference between the activated microglial numbers between untreated wt and AAV+BMT-mut groups. The horizontal bars represent means, and the error bars represent SEM. *p < 0.05, **p < 0.01.