Table 3. Associations between SNPs belonging to NOS2 and NOS3 and two quantitative phenotypes measured in the exhaled breath condensate (FeNO level and NO2–NO3 level) in non-asthmatic and asthmatic subjects–Single marker analysis.
Exhaled phenotypes | |||||||||||||
FeNO, ppb | NO2-NO3, µmol/mg | ||||||||||||
Chromosome | Gene | Genotyping method | Marker | Position (bp) | Major allele* | Minor allele | MAF† | beta | 95% CI # | P | beta | 95% CI # | P |
Non-asthmatic subjects | |||||||||||||
17 | NOS2 ‡ | 610K | rs4795067 | 23130802 | A | G | 0.35 | −0.04 | −0.10; 0.03 | 0.26 | 0.13 | 0.04; 0.22 | 0.002 |
610K | rs6505510 | 23203917 | A | C | 0.40 | 0.10 | 0.04; 0.17 | 0.002 | 0.08 | −0.01; 0.17 | 0.07 | ||
7 | NOS3 ‡ | Taqman | rs1549758 | 150326659 | C | T | 0.35 | −0.06 | −0.10; −0.02 | 0.0009 | 0.003 | −0.06; 0.06 | 0.92 |
Taqman | rs2853796 | 150334848 | G | T | 0.48 | 0.06 | 0.02; 0.09 | 0.0007 | −0.02 | −0.08; 0.04 | 0.46 | ||
Asthmatic subjects | |||||||||||||
7 | NOS3 ‡ | 610K | rs743507 | 150338421 | T | C | 0.27 | 0.08 | 0.03; 0.14 | 0.004 | 0.02 | −0.07; 0.11 | 0.65 |
In bold P-values surviving the adjustment (P≤0.0024 or P≤0.01 for NOS2 and NOS3 respectively).
Major allele was consider as baseline allele
MAF: Minor allele frequency
CI: confidence interval
Analyses were conducted under an additive genetic model for NOS3 SNPs and under recessive or dominant genetic models for NOS2 SNPs and FeNO and EBC NO2–NO3 levels respectively.