Figure 1. Contact inhibition disrupted by EGTA followed by EGF+FGF-2 leads to EMT.

ARPE-19 cells cultured to day 7 post-confluence were treated with PBS, 1 mM EGTA, or 1 mM EGTA plus 10 ng/ml EGF and 20 ng/ml FGF-2 for 1 day. Immunostaining to RPE65, N-cadherin, ZO-1, and Na, K-ATPase was detected in the intercellular junction in PBS and EGTA, but was notably diminished in EGTA+EGF+FGF-2. Positive staining to S100A4 and α-SMA emerged. Scale bar indicates 100 μm.