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. 2012 May 9;7(5):e36864. doi: 10.1371/journal.pone.0036864

Figure 2. Contact inhibition is unlocked without EMT by p120 knockdown.

Figure 2

ARPE-19 cells cultured to post-confluence day 7 were transfected with 100 nM scRNA or siRNAs to such junctional components as p120, N-cadherin, α-catenin, β-catenin, and ZO-1 for 2 days, and cultured in the presence of 10 μm BrdU for 4 h before fixation in acid/methanol. (A) DIC cell morphology was not altered by any siRNA compared to the scRNA control. BrdU nuclear labeling was only increased in cells transfected with p120 siRNA, where some were colocalized with nuclear translocation of p120. (B) Immunostaining of RPE65, N-cadherin, ZO-1, Na,K-ATPase, S100A4, and α-SMA in cells transfected with p120 siRNA was similar to that of the scRNA control. Scale bar indicates 100 μm and * denotes P<0.05.