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. 2012 May 9;7(5):e36843. doi: 10.1371/journal.pone.0036843

Figure 6. Intensity distribution profiles of ACBD6 and ZNF23 in Ct infected cells.

Figure 6

HeLa cells were grown on coverslips and infected in cell culture medium without antibiotics and cycloheximide. Results obtained for ACBD6 in cells infected with Ct strain D 24 hr post-infection, and for ZNF23 in cells infected with strain E 18 hr post-infection, are shown in panel A and B, respectively. The images were analyzed with ZEN 2010 software (Zeiss LSM710). Traces of the intensity of the signal (y axis) were plotted in function of the distance (x axis) of the section of the cell indicated by a red arrow (from left to right). Panel A shows the merged image of ACBD6 (green), LPS (red) and DNA (blue) in a cell with two inclusions. Panel B displays the merged image of the signal for ZNF23 (green) and DNA (blue). Note that the Ct inclusion (Inc) can be detected with the Hoechst dye, which also stained bacterial DNA.