Figure 8. Expression of host proteins recruited to the inclusion is not altered in Ct infected cells.

HeLa cells were grown in 24-well plates in cell culture medium without antibiotics and cycloheximide. Cells were infected at a MOI of 5 with Ct strain D (panel A. middle graph and panel B) and with L₂ (panel A, right graph and panel C). Some cells were not infected (panel A, left graph). At the indicated time, total RNAs were isolated and cDNAs were synthesized with random primers. qPCR analysis was performed as described in the Method section. Expression levels of the human genes ACBD3, ACBD6, ACSL3, LPCAT1 and ZNF23 (panel A) were normalized to levels of GAPDH gene. In cells infected with Ct D (panel B) or with Ct L₂ (panel C), Ct genes incA, incG and ompA were normalized to expression levels of the euo gene. For each gene, expression level ratio obtained at time point 0 hr was arbitrary set at a value of 1 and all other ratios were calculated relative to it. Note the difference of the x axis scale of the 3 plots in panel A compare to the graph in panel B and C.