Table S6.

TCS domains in several organisms.

Organismus	Mist-annotation/ScanProsite or SMART count1
	HisKa	Response reg
E. coli K-12	29/77	31/39
Staphylococcus aureus (STAAN)	18/30	17/285
Listerien monocytogenes (LISMO) EGD	16/56	16/54
Arabidospis thaliana (ARATH)	16/61	22/285
Zea mays (MAIZE)	20/25	22/44

Notes:

¹The Table compares the annotated number of TCS domains in MIST database that are known to belong to TCS versus the TCS domains found by motif similarity using ScanProsite or domain similarity using SMART. The two plant examples are not yet annotated in MIST, however, for these organisms there are in Arabidopsis 16 His protein kinases (Hwang et al, Plant Physiology 2002, 129:500–515) and 22 response regulators (ARRs), 12 of which contain a Myb-like DNA binding domain called ARRM (type B). The remainder (type A) possess no apparent functional unit other than a signal receiver domain containing two aspartate and one lysine residues (DDK) at invariant positions, and their genes are transcriptionally induced by cytokinins without de novo protein synthesis. The type B members, ARR1 and ARR2, bind DNA in a sequence-specific manner and work as transcriptional activators (Database of Arabidopsis transcription factors, http://datf.cbi.pku.edu.cn/browsefamily.php?familyname=GARP-ARR-B). In Maize there are 11 cytokinin receptory, 9 phosphotransfer proteins and 22 response regulators (Chu et al, Genet Mol Res. 2011;10(4):3316–3330).