Figure 1. Assembly Kinetics of E7SOs followed by different spectroscopy probes.

(A) CD spectra obtained at different times during E7SOs assembly. CD spectrum of E7₂ before addition of EDTA (full line) and CD spectra of E7 at different times after EDTA addition, at 3 minutes (dashed line), 15 minutes (full line with rectangles) and at 60 minutes (dashed and dotted line). (B) Kinetics of E7SOs assembly monitored by circular dichroism and tyrosine fluorescence. Far-UV CD at 216 nm (black circles) and tyrosine fluorescence (full line); both experiments were carried out at the same protein concentration and triggered by the addition of 1.0 mM EDTA. (C) Kinetics of E7SOs assembly followed by Thioflavin T fluorescence (black line) and ANS fluorescence (grey line); both experiments were carried out at the same protein concentration and triggered by the addition of 1.0 mM EDTA right before beginning the measurement.